Products

The one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths. Benefits of the BioFX™ family of substrates include: Prolonged stability Increased sensitivity Negligible backgrounds Ease of manufacturing

Cushioned primary envelope for Petaka transport

StemBeads® FGF2 is a patented growth factor supplement that offers a novel way to culture cells with native Fibroblast Growth Factor 2 more efficiently. FGF2 is key for growth of human induced pluripotent stem cells (iPSCs), fibroblast cells, wound healing cells such as macrophages, cancer cells, and neural progenitor cells (NPCs). StemBeads® FGF2 are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with native recombinant human FGF2. Controlled delivery and stable levels overcome the 4.5 hour half-life (Figure below) of FGF2 and improve cell cultures while saving researchers valuable time and resources.

Anti-Mouse Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for mouse immunoglobulins. Minimal cross reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [1]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 & 15-1117). For detailed instructions for use, see EpiCypher’s CUTANA™ CUT&Tag protocol: epicypher.com/protocols. *Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.

Human histone octamers (two each of histones H2A, H2B, H3 and H4) are made from recombinant histones expressed in E. coli. Octamers are the protein component of nucleosomes, the basic subunit of chromatin. Assembling this product with DNA bearing a 601 Widom sequence (e.g. EpiCypher 18-0005, 18-0006) produces a nucleosome. The 601 Widom sequence, originally discovered by Lowary and Widom [1], has high affinity for octamers.

SiR-tubulin is based on the microtubule binding drug Docetaxel. SiR-tubulin is fluorogenic, cell permeable and highly specific for microtubules. Sir-tubulin stains endogenous microtubules without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-tubulin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-tubulin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.

SiR-DNA (SiR-Hoechst*) is a live cell nuclear stain based on the DNA minor groove binder bisbenzimide, it allows the labelling of DNA in live cells with high specificity and low background. SiR-DNA is fluorogenic, cell permeable and highly specific for DNA. Sir-DNA stains the nuclei of live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-DNA is compatible with GFP and/or m-cherry fluorescent proteins. SiR-DNA is a non cytotoxic alternative to DRAQ5. It can be imaged with standard Cy5 filtersets. SiR-DNA can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.