Results for Proteins & Peptides ( 104972 )
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The wild-type human G12D+I36N K-Ras4B protein has been produced in a bacterial expression system. The recombinant protein contains six histidine residues at its amino terminus (His-tag). The molecular weight of 6xHis tagged G12D+I36N K-Ras4B is approximately 25 kDa and is supplied as a white lyophilized powder. Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gradient gel. His tagged G12D+I36N K-Ras4B protein was determined to be >90% pure. Applications for CS-RS10 include: Study of G12D+I36N K-Ras4B exchange activity with different GEFs, Identification of G12D+I36N K-Ras4B exchange factors (GEFs), Positive control for GEF studies, Biochemical characterization of G12D+I36N K-Ras4B protein interactions and as a Western blot standard