Proteins & Peptides

Mutant version of our regular EZH2 5-member complex (Cat. # 51004), but with an Ala-to-Thr mutation on a.a. 738 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), (a-a 2-end ; A738T*) with N-terminal His tag, MW= 86 kDa, human EED (NM_003797) (a-a 2-end) with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355) (a-a 2-end) with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207) (a-a 2-end) with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610) (a-a 2-end) with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system.

Human SUV39H1, GenBank Accession No. NM_003173, a.a. 82-412, with N-terminal GST-tag, MW= 64 kDa, expressed in an E. coli expression system.

Human SUV39H2, GenBank Accession No. NM_024670, a.a. 26-350, with N-terminal GST-tag, MW= 63 kDa, expressed in an E. coli expression system.

Human SUV4-20H1 (GenBank Accession No. NM_017635), a.a. 2-387, with N-terminal GST-tag, MW= 70 kDa, expressed in an E. coli expression system.

Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-GST-tag, MW= 114 kDa, human SUZ12 (NM_015355), a.a. 1-end with N-terminal His-tag, MW = 87 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His-tag, MW= 48 kDa. Co-expressed in a baculovirus expression system.

Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His-tag, MW = 87 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.

Recombinant human DNMT1 (DNA methyltransferase 1, isofrom b), encompassing amino acids 501-1632 (end). This construct contains an N-terminal His-tag (6xHis) followed by a FLAG tag. The protein was affinity purified.

Recombinant human DNMT1 (DNA methyltransferase 1, isoform b ), full-length, encompassing amino acids 2-1632(end). This construct contains an N-terminal GST-tag and includes a thrombin cleavage site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

Human DNMT2 (GenBank Accession No. NM_004412), (a.a. 2-391(end)), with N-terminal GST-tag, MW=71 kDa, expressed in baculovirus expression system.