Proteins & Peptides

Akt is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3) at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.

NFkB p65 phospho S276 peptide for use in control assays with purified antibodies Anti-NFkB p65 pS279 (p/n 100-401-264 and 600-401-264). Also available NFkB p65 non-phospho S276 peptide (p/n 000-000-264NP).

NFkB p65 non-phospho S276 peptide for use in control assays with purified antibodies Anti-NFkB p65 pS279 (p/n 100-401-264 and 600-401-264). Also available NFkB p65 phospho S276 peptide (p/n 000-000-264).

NFkB p65 phospho S529 peptide for use in control assays with purified antibodies Anti-NFkB p65 pS529 (p/n 100-401-266). Also available NFkB p65 non-phospho S529 peptide (p/n 000-000-266NP).

NFkB p65 non-phospho S529 peptide for use in control assays with purified antibodies Anti-NFkB p65 pS529 (p/n 100-401-266). Also available NFkB p65 phospho S529 peptide (p/n 000-000-266).

ATM non-phospho S1981 peptide for use in control assays with purified antibody Anti-ATM S1981 non-phospho (p/n 600-401-398). Also available ATM phospho S1981 peptide (p/n 000-000-400) and Anti-ATM pS1981 phospho antibodies (p/n 200-301-400 and 600-601-400).

ATM phospho S1981 peptide for use in control assays with purified antibodies Anti-ATM pS1981 (p/n 200-301-400 and 600-601-400). Also available ATM non-phospho S1981 peptide (p/n 000-000-398) and Anti-ATM non-phospho S1981 (p/n 600-401-398).

Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodies, but can also occur with monoclonals as well. To determine which band or staining is specific, the AKT immunizing peptide blocking experiment can be performed. Prior to the staining protocol, the anti-AKT antibody is neutralized (incubated with an excess of peptide that corresponds to the epitope recognized by the antibody). The AKT antibody that is bound to the blocking peptide is no longer available to bind to the epitope present in the protein on the Western blot or in the cell. The neutralized antibody is then used side-by-side with the antibody alone, and the results are compared. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is specific: this staining will be absent from the Western blot or immunostaining performed with the neutralized antibody.

This peptide is a control blocking and immunogen peptide for the cardiovascular angiopoietin antibodies 100-401-402 and 100-401-402S