Proteins & Peptides

Human Fibroblast Growth Factor Receptor 3 (FGFR3), also known as JTK4, and Hydroxyaryl-Protein Kinase with V555F mutation, GenBank Accession No. NM_000142, a.a. 447-761, with N-terminal His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system, phosphorylation level <5%. MW = 37 kDa.

Mouse transforming growth factor beta receptor 2 (TGFβR2), also known as TGFBR2, TGFR-2, and TGF-Beta Receptor Type 2, GenBank Accession No. NM_009371, a.a. 24-184 fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system, MW = 47 kDa. This protein runs at a higher molecular weight due to glycosylation.

Transglutaminase from K. albica, Genbank accession # AHI00814.1, a.a. 29-262(end) with a C-terminal His-tag, expressed in E. coli. MW = 28 kDa

Human Angiotensin converting enzyme 2 (ACE2) also known ACEH, GenBank Accession No. NP_068576.1, a.a. 18-740, with a C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=85 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Complex of Human JAK2, also known as Janus Kinase 2, with V617F, Y1007F and Y1008F mutations, GenBank Accession No. NM_004972, a.a. 36-1132(end), with N-terminal FLAG-tag, MW=128 kDa, and human EPOR, also known as Erythropoietin receptor, GenBank Accession No. NM_000121, a.a. 273-338 with N-terminal His-tag, MW=9 kDa. The proteins were expressed as single fusion protein with a (GSSG)₈ linker in a Baculovirus infected Sf9 cell expression system.

Complex of Human EPOR, also known as Erythropoietin receptor, GenBank Accession No. NM_000121, a.a. 273-338 with N-terminal His-tag, MW=9 kDa, and JAK2, also known as Janus Kinase 2, with V617F, Y1007F and Y1008F mutations, GenBank Accession No. NM_004972, a.a. 36-1132(end), with N-terminal FLAG-tag, MW=128 kDa. The proteins were expressed as single fusion protein with a (GSSG)₆ linker in a Baculovirus infected Sf9 cell expression system.

Recombinant human ALK2 (Activin receptor-like kinase 2) encompassing amino acids 147-509 (end) and containing the clinically relevant mutation R206H. In this construct the N-terminal end of ALK2 is fused with GST. A short peptide linker placed between GST and PARP7 includes a thrombin site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

Severe acute respiratory Coronavirus 2 RNA dependent RNA polymerase, GenBank Accession No. YP_00972530, a.a. 1-932(full length), with an N-terminal FLAG-tag expressed in a HEK293 expression system. MW=108 kDa.

Human Cluster of Differentiation 19 (CD19), also known as B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, GenBank Accession No. NM_001770, a.a 21-291, with C-terminal Avi-Tag™ fused to the Fc portion of Human IgG1 and labeled with phycoerythrin (PE), expressed in a HEK293 cell expression system. MW = 59 kDa. This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.