Proteins & Peptides

The Human Thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on SDS-PAGE. MW = 37 kDa.

The Human Factor Xa was activated from Human Factor X by activation with Russell's Viper Venom. This RVV-X was removed after activation. Complete activation is observed on SDS-PAGE. MW = 46 kDa.

Human Caspase 9 (CASP9), GenBank Accession No. NM_001229, a.a. 1 - 416 (end), with C-terminal His-tag, MW=35 &10 kDa, expressed in an E. coli cell expression system. Procaspase-9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product contains both the large (p35) and small (p10 [His-tag]) protease subunits.

Human apoptotic peptidase activating factor 1 (APAF1), also known as CED4 (GenBank Accession No. NM_013229), a.a. 2-1237(end) with N-terminal His-tag and N-terminal FLAG-tag, MW = 143 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Human NLR Family Pyrin Domain Containing 3 (NLRP3), also known as NALP3, Cold Autoinflammatory Syndrome 1 Protein (CIAS1), cryopyrin and PYPAF1 (Genbank Accession No. NM_004895), a.a. 2-1036(end) with N-terminal His-FLAG-tag, expressed in a baculovirus infected Sf9 cell expression system. MW = 120 kDa (calculated).

Human Activated Renin (GenBank Accession No. NM_000537), amino acids 67 - 406 (end) with C-terminal HIS tag, MW=38.6 kDa, expressed in a mammalian expression system.

Rat, Activated Renin (GenBank Accession No. NM_012642), a.a. 65 - 402 (end) with C-terminal His-tag, MW=38.5 kDa, expressed in an HEK293 cell expression system.

Human membrane metallo-endopeptidase (MME), also known as CALLA, neutral endopeptidase (NEP), CD10, and Neprilysin (GenBank Accession No. NM_000902.3), a.a. 52-750(end), with N-terminal His-tag, MW= 81kDa, expressed in HEK293 cell expression system.

Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system.