Proteins & Peptides

Human LIGHT protein, also known as TNFSF14, GenBank Accession No. NM_003807, a.a. 74-240 with N-terminal His-tag. MW = 21 kDa, expressed in Sf9 cells via a baculovirus expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Mouse secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_008798, a.a 25-167, fused at the C-terminus to the Fc portion of mouse IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.

Human Secreted Programmed Cell Death Ligand 1 (PD-L1)-Fc fusion protein, also known as, CD47 or B7-H1, GenBank Accession No. NM_021893, a.a 18-238, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 51 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.

Human secreted CD58-Fc fusion protein, also known as LFA-3, GenBank Accession No. NM_001779, a.a. 29-215, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

Human CD38, also known as cluster of differentiation 38 and cyclic ADP ribose hydrolase with C-terminal His-tag, GenBank Accession No. NM_001775, a.a. 43-300 expressed in a HEK293 cell expression system. MW = 31 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Human Programmed Cell Death 1 Ligand 1 (PD-L1), also known as CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1, GenBank Accession No. NM_014143, a.a. 19-239, with a C-terminal FLAG-Avi-His-tag, expressed in a HEK293 cell expression system. MW = 30 kDa.

Human CD39, also known as Cluster of Differentiation 39, GenBank Accession number NM_001776, a.a. 38-478 with C-terminal His-tag. MW = 51 kDa, expressed in an HEK293 expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Mouse FcRn also known as Neonatal Fc receptor is a heterodimer of FCGRT (Fc Fragment of IgG Receptor and Transporter) and B2M (beta 2 microglobulin), GenBank Accession numbers NM_010189 and NM_009735; a.a. 22-297 and a.a. 1-119 respectively. FCGRT has a C-terminal His-Avi-tag. This protein is enzymatically biotinylated using AviTagTM technology. These proteins are co-expressed in a HEK293 expression system. FcRn MW = 34 kDa, B2M MW = 14 kDa.

Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, integrin-associated protein (IAP), OA3, and MER6, GenBank Accession No. NM_001777, a.a. 19-139, with C-terminal Avi-Tag, expressed in a HEK293 cell expression system. The purified protein (40 kDa) was covalently labeled by streptavidin (~13 kDa). Protein runs at a higher MW by SDS-PAGE due to glycosylation and streptavidin labeling.