Proteins & Peptides

Human nitric oxide synthase 3 (NOS3), also known as endothelial NOS (eNOS), (GenBank Accession No. NM_000603), a.a. 2 - 1203 (end) with N-terminal His-FLAG-tag, MW= 135 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 - 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.

Human Peroxiredoxin I (GenBank Accession No. NM_002574), the full length protein containing a Lysine to Glutamine mutation at position 197 and an N-terminal His-tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human Peroxiredoxin I (GenBank Accession No. NM_002574), the full length protein containing a Lysine to Arginine mutation at position 197 and an N-terminal His-tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with N-terminal His-FLAG tag, MW = 37.1 kDa, expressed in an E. coli cell expression system.

Biotin ligase BirA, GenBank Accession No. NP_418404, a.a. 2 - 321 with Nterminal GST-tag, MW = 61 kDa, expressed in an E. coli cell expression system.

Biotin ligase BirA, GenBank Accession No. AP012306, a.a. 2 - 321 (end) with N-terminal His-FLAG tag, MW = 37 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Human Receptor Activator of Nuclear Factor κB (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation

Staphylococcus aureus sortase A Pentamutant (P94R, D160N, D165A, K190E, K196T), a.a. 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 21.7 kDa, expressed in an E. coli expression system.