Results for Nucleic Acids ( 3145 )
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Nucleosome Assembly 601 Sequence DNA is a 147 base-pair double-stranded DNA fragment that was identified by Lowary and Widom using the SELEX method [1]. The 601 Sequence DNA has high affinity for histone octamers and is useful for in vitro nucleosome assembly. The DNA contains a 5’ biotin-TEG group.
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Fragmented DNA derived from Escherichia coli (E. coli) can be used as a spike-in control for experimental normalization in Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUT&RUN normalization was originally performed with carryover E. coli DNA from MNase preparation [1], however pure preps of enzyme require post hoc DNA spike-in. CUTANA™ E. coli Spike-in DNA contains sufficient material for 100-200 CUT&RUN reactions (range for high and low abundance targets).